B.Sc. BBZ(Biochemistry,Botany, Zoology)

Programme/Class: DEGREE

Year: THIRD

Semester: FIFTH

Subject: Biochemistry

Course Code: B110502 T

Course Title  Fundamentals of Microbiology

Course outcomes:After the successful course completion, learners will develop following attributes

·         Know the basics of microbiology

·         Have knowledge of the general classification of microbes

·         understand basics of Control of Microorganisms

·         Study microbes in extreme environments and microbial interactions

·         Know the basics of recombination in Prokaryotes

·         Food & Industrial Microbiology

·         Basics of virology

Credits: 4

Core Compulsory

Max. Marks: 25+75

Min. Passing Marks:

Total No. of Lectures (in hours per week):

Unit

Topics

No. of Lectures (60)

I

History of Microbiology

·         Spontaneous generation versus biogenesis

·         Contributions of Anton von Leeuwenhoek, Louis Pasteur, Robert Koch, Alexander Fleming

·         Various forms of microorganisms (bacteria, fungi, viruses, protozoa, PPLOs)

4

II

Classification of microbiology

·         Nutritional classification of microorganisms

·         Nature of the microbial cell surface

·         Gram positive and Gram negative bacteria

·         Growth curve

8

III

Control of Microorganisms

·         Physical agents (Autoclave, Hot air oven, Laminar airflow and membrane filter.)

·         Chemical agents (Alcohol, Halogens and Gaseous agents antibiotics), Radiation Methods (UV rays)

8

IV

Pathogenicity of Microorganisms and Antimicrobial Chemotherapy

·         Introduction to pathogenic microbes, Bacteria, Viruses, Algae, protozoa and fungi

·         General Characteristics of antimicrobial drugs

·         determining the level of microbial activity

·         dilution susceptibility test and disc diffusion test

·         Range of activity and mechanism of action of penicillin, vancomycin and tetracycline.

8

V

Microbes in extreme environments and microbial interactions

·         The thermophiles alkalophiles, acidophiles

·         symbiosis and antibiosis among microbial population

·         N2 fixing microbes in agriculture and forestry.

8